Difference between revisions of "Part:BBa K177025"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K177025 short</partinfo>
 
<partinfo>BBa_K177025 short</partinfo>
  
  
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This device was constructed as a test to determine which of the E. coli strains available were truly araC<sup>-</sup>.
  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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When tested strains transformed only with this brick are incubated with arabinose and no green fluorescence is observer then the bacteria are araC<sup>-</sup>.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K177025 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K177025 SequenceAndFeatures</partinfo>

Revision as of 15:25, 21 October 2009

GFP under araC promotor


This device was constructed as a test to determine which of the E. coli strains available were truly araC-.


Usage and Biology

When tested strains transformed only with this brick are incubated with arabinose and no green fluorescence is observer then the bacteria are araC-.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1013
    Illegal SapI site found at 107