Difference between revisions of "Part:BBa K257002"
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ClyA (or HlyE) is an alpha-PFT for Pore Forming Toxins.  
 
ClyA (or HlyE) is an alpha-PFT for Pore Forming Toxins.  
  
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PFTs are potent virulence factors class starting in a soluble form to an outer membrane-integrated pore. They exhibit their toxic effect either by membrane permeability barrier destruction or by toxic components delivery through the pores which forming by several assembly 8 or 13 ClyA subunits.
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*ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs
 +
*We can fuse GFP to the C or N term of Cly A, to track OMVs easily.
 +
*ClyA is capable of co-localizing a variety of structurally diverse fusion partners to the surface of E. coli and their released vesicles, but only when the periplasmic disulfide bond-forming machinery was present ,it’s makes OMVs an ideal structure to transport hydrophobic compounds like membrane proteins into the host.
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*Cly A confers vesicle binding to and invasion of host cells.
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*ClyA was significantly enriched in OMVs relative to other lumenal and membrane bound OMV proteins. <!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
−
PFTs are potent virulence factors class starting in a soluble form to an outer membrane-integrated pore. They exhibit their toxic effect either by membrane permeability barrier destruction or by toxic components delivery through the pores which forming by several assembly 8 or 13 ClyA subunits.
 
  
−
#ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs
 
−
#We can fuse GFP to the C or N term of Cly A, to track OMVs easily.
 
−
#ClyA is capable of co-localizing a variety of structurally diverse fusion partners to the surface of E. coli and their released vesicles, but only when the periplasmic disulfide bond-forming machinery was present ,it’s makes OMVs an ideal structure to transport hydrophobic compounds like membrane proteins into the host.
 
−
#Cly A confers vesicle binding to and invasion of host cells.
 
−
#ClyA was significantly enriched in OMVs relative to other lumenal and membrane bound OMV proteins.
 
  
 
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Revision as of 15:23, 21 October 2009

ClyA_Fusion_C-Term

ClyA (or HlyE) is an alpha-PFT for Pore Forming Toxins.

PFTs are potent virulence factors class starting in a soluble form to an outer membrane-integrated pore. They exhibit their toxic effect either by membrane permeability barrier destruction or by toxic components delivery through the pores which forming by several assembly 8 or 13 ClyA subunits.

  • ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs
  • We can fuse GFP to the C or N term of Cly A, to track OMVs easily.
  • ClyA is capable of co-localizing a variety of structurally diverse fusion partners to the surface of E. coli and their released vesicles, but only when the periplasmic disulfide bond-forming machinery was present ,it’s makes OMVs an ideal structure to transport hydrophobic compounds like membrane proteins into the host.
  • Cly A confers vesicle binding to and invasion of host cells.
  • ClyA was significantly enriched in OMVs relative to other lumenal and membrane bound OMV proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]