Difference between revisions of "Part:BBa K5387000:Design"
Line 5: Line 5:
 
: fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'  
 
: fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'  
 
: rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'
 
: rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'
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===Source===
 
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This gene is expressed in humans KÄLLA
 
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===References===
 

Revision as of 19:00, 21 September 2024

Design Notes

The sequence has been optimized for expression in E. coli.

Primers for cloning into pET28a(+) between restriction sites NdeI and EcoRI:

fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'