Difference between revisions of "Part:BBa K5036030"

Revision as of 17:45, 21 September 2024

dCas9(N)_NES-Syn-VEGFR-2 (VEGF-R2, N-TEV, NES, TCS (Q, G), HA, dCas9(N),mCherry)

Part Description

In our second receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R2, is attached to an internal domain composed of N terminal domain of TEV protease, a nuclear export signal (NES), a TEV cleavage site(TCS(Q,G)), and dCas9(N).

Usage

this is our receptor's second chain. our receptor is activated after binding of VEGF to the external domain which is designed to attach specifically to it. after activation the two domains of TEV dimerizes forming catalytically active TEV protease which will cleave the two chains at TCS. upon cleavage of the two chains the two domains of dCas9 dimerize and is released attached to transcription activator to be guided to its direction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 982
Illegal BglII site found at 2341
Illegal BglII site found at 3647
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4474
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1854
Illegal BsaI.rc site found at 664
Illegal BsaI.rc site found at 1442
Illegal BsaI.rc site found at 2731
Illegal SapI.rc site found at 3289

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K5036030 short</partinfo>
+==Part Description==
-In our second receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R2, is attached to a protein sequence containing TEV protease, a nuclear export signal (NES), a TEV cleavage site(TCS), and dCas9(N). When an injury occurs, VEGF levels rise outside the cell. This binds to VEGF-R2, activating the receptor and triggering the TEV protease to cleave the chain at the TCS site. This releases dCas9(N), which can then associate with a complementary dCas9 fragment (dCas9(C)) to form a fully functional dCas9 enzyme
+In our second receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R2, is attached to an internal domain composed of N terminal domain of TEV protease, a nuclear export signal (NES), a TEV cleavage site(TCS(Q,G)), and dCas9(N).
+==Usage==
+this is our receptor's second chain. our receptor is activated after binding of VEGF to the external domain which is designed to attach specifically to it. after activation the two domains of TEV dimerizes forming catalytically active TEV protease which will cleave the two chains at TCS. upon cleavage of the two chains the two domains of dCas9 dimerize and is released attached to transcription activator to be guided to its direction.