Difference between revisions of "Part:BBa K5321007:Design"

 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K5321007 short</partinfo>
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<partinfo>BBa_K5321006 short</partinfo>
  
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<partinfo>BBa_K5321007 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5321006 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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e
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Gene synthesis should be performed to obtain this coding sequence.
  
  
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===Source===
 
===Source===
  
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e
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Nucleotide sequence reconstruction was performed using the amino acid sequences given in the Supplementary Material of Tina Fink et al., 2019, optimized for codon optimization in E. coli.
  
 
===References===
 
===References===

Latest revision as of 15:15, 21 September 2024


Plum pox virus protease (PPVp), codon optimized for E. coli, 6x His tagged


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 712
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 712
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 712
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene synthesis should be performed to obtain this coding sequence.


Source

Nucleotide sequence reconstruction was performed using the amino acid sequences given in the Supplementary Material of Tina Fink et al., 2019, optimized for codon optimization in E. coli.

References