Difference between revisions of "Part:BBa K177002"
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<partinfo>BBa_K177002 short</partinfo> | <partinfo>BBa_K177002 short</partinfo> | ||
− | Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is | + | Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is artificially designed (based on natural fragment of lambda phage genome) to increase the affinity for cro protein. |
+ | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | When this part is introduced into a sequence of elements expressed from a single promoter the binding of the Cro protein (<partinfo>K177009</partinfo>) successfully inhibits the expression of elements located downstream. | ||
+ | |||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K177002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K177002 SequenceAndFeatures</partinfo> |
Revision as of 14:51, 21 October 2009
cro binding box
Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is artificially designed (based on natural fragment of lambda phage genome) to increase the affinity for cro protein.
Usage and Biology
When this part is introduced into a sequence of elements expressed from a single promoter the binding of the Cro protein (BBa_K177009) successfully inhibits the expression of elements located downstream.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]