Difference between revisions of "Part:BBa K216007:Experience"
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===Applications of BBa_K216007=== | ===Applications of BBa_K216007=== | ||
| − | Lumazine protein (LumP) of ''Photobacterium phosphoreum'' is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In ''P. phosphoreum'', it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of ''P. phosphoreum'' luminescence compared to other bioluminescent bacteria such as ''Xenorhabdus luminescens''. To find out, we hope to compare bioluminescence of ''E. coli'' expressing ''X. luminescens luxAB'' (BBa_K216008) with and without lumazine protein. | + | Lumazine protein (LumP) of ''Photobacterium phosphoreum'' is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In ''P. phosphoreum'', it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (Lee et al, 1991; O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of ''P. phosphoreum'' luminescence compared to other bioluminescent bacteria such as ''Xenorhabdus luminescens''. To find out, we hope to compare bioluminescence of ''E. coli'' expressing ''X. luminescens luxAB'' (BBa_K216008) with and without lumazine protein. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:50, 21 October 2009
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Applications of BBa_K216007
Lumazine protein (LumP) of Photobacterium phosphoreum is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In P. phosphoreum, it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (Lee et al, 1991; O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of P. phosphoreum luminescence compared to other bioluminescent bacteria such as Xenorhabdus luminescens. To find out, we hope to compare bioluminescence of E. coli expressing X. luminescens luxAB (BBa_K216008) with and without lumazine protein.
User Reviews
UNIQ16f398c2ca02e87f-partinfo-00000000-QINU UNIQ16f398c2ca02e87f-partinfo-00000001-QINU