Difference between revisions of "Part:BBa K216007:Experience"

Revision as of 14:50, 21 October 2009

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Applications of BBa_K216007

Lumazine protein (LumP) of Photobacterium phosphoreum is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In P. phosphoreum, it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of P. phosphoreum luminescence compared to other bioluminescent bacteria such as Xenorhabdus luminescens. To find out, we hope to compare bioluminescence of E. coli expressing X. luminescens luxAB (BBa_K216008) with and without lumazine protein.

User Reviews

UNIQ01ef74630631a579-partinfo-00000000-QINU UNIQ01ef74630631a579-partinfo-00000001-QINU

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 ===Applications of BBa_K216007===
-Lumazine protein (LumP) of ''Photobacterium phosphoreum'' is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In ''P. phosphoreum'', it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of ''P. phosphoreum'' luminescence compared to other bioluminescent bacteria such as ''Xenorhabdus luminescens''. To find out, we hope to compare bioluminescence of ''E. coli'' expressing ''X. luminescens luxAB'' (BBa_K216008) with and without lumazine protein. At the time of writing, days before the freezing of the iGEM 2009 information for judging, we have not yet been able to perform this exciting experiment, but we hope to post the results here in the near future (possibly shortly after the Jamboree).
+Lumazine protein (LumP) of ''Photobacterium phosphoreum'' is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In ''P. phosphoreum'', it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). It may be partially responsible for the much higher perceived brightness of ''P. phosphoreum'' luminescence compared to other bioluminescent bacteria such as ''Xenorhabdus luminescens''. To find out, we hope to compare bioluminescence of ''E. coli'' expressing ''X. luminescens luxAB'' (BBa_K216008) with and without lumazine protein.
 ===User Reviews===