Difference between revisions of "Part:BBa K5396008"
Line 7: Line 7:
  
 
===Usage and Biology===
 
===Usage and Biology===
 +
texto sobre a BARBIE e sobre a importancia da cisteina
  
 
===Part Generation===
 
===Part Generation===

Revision as of 16:12, 20 September 2024


T7-Barbie1-Cys

This composite part codes for the BARBIE1 protein with an additional amino acid (cysteine)(BBa_K53960004). This enhancement allows it to be effectively utilized in our biosensor technology.

This part consists of the following basic parts: BBa_R0010, BBa_J435345, BBa_K53960004 and BBa_J428069.

Usage and Biology

texto sobre a BARBIE e sobre a importancia da cisteina

Part Generation

Golden Gate

Expression and purification

Testing

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
    Illegal AgeI site found at 344
  • 1000
    COMPATIBLE WITH RFC[1000]