Difference between revisions of "Part:BBa K5396008"
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<partinfo>BBa_K5396008 short</partinfo>
 
<partinfo>BBa_K5396008 short</partinfo>
  
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BARBIE1 is a synthetic protein derived from BaCBM2 (BBa_K5396003) through a process of reverse engineering. It has the increased ability to bind to plastics when compared to BaCBM2.
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This composite part codes for the BARBIE1 protein with an additional amino acid (cysteine)(BBa_K53960004). This enhancement allows it to be effectively utilized in our biosensor technology.
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<p>This part consists of the following basic parts: BBa_R0010, BBa_J435345, BBa_K53960004 and BBa_J428069.</p>
  
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This BARBIE1 protein is modified with an additional amino acid (cysteine). This enhancement allows it to be effectively utilized in our biosensor technology.
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===Usage and Biology===
  
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===Cloning===
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===Part Generation===
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Golden Gate
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===Expression and purification===
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===Testing===
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:07, 20 September 2024


T7-Barbie1-Cys

This composite part codes for the BARBIE1 protein with an additional amino acid (cysteine)(BBa_K53960004). This enhancement allows it to be effectively utilized in our biosensor technology.

This part consists of the following basic parts: BBa_R0010, BBa_J435345, BBa_K53960004 and BBa_J428069.

Usage and Biology

Part Generation

Golden Gate

Expression and purification

Testing

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
    Illegal AgeI site found at 344
  • 1000
    COMPATIBLE WITH RFC[1000]