Difference between revisions of "Part:BBa K5396011"

 
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<partinfo>BBa_K5396006 short</partinfo>
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<partinfo>BBa_K5396011 short</partinfo>
  
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This is the spidroin protein fusioned with BARBIE1 protein.  
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This composite part codes for the N terminal of Nt2RepCt fused with BARBIE1 protein (BBa_K53960010).
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<p>This part consists of the following basic parts: BBa_J435350, BBa_J435345, BBa_K53960010 and BBa_J428069.</p>
  
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<!-- Add more about the biology of this part here
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===Part Generation===
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===Usage and Biology===
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Gibson Assembly
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===Expression and purification===
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===Testing===
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5396006 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5396011 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K5396006 parameters</partinfo>
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<partinfo>BBa_K5396011 parameters</partinfo>
 
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Revision as of 11:46, 20 September 2024


T7-Nt-Barbie1-Cys

This composite part codes for the N terminal of Nt2RepCt fused with BARBIE1 protein (BBa_K53960010).

This part consists of the following basic parts: BBa_J435350, BBa_J435345, BBa_K53960010 and BBa_J428069.

Part Generation

Gibson Assembly

Expression and purification

Testing

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
    Illegal AgeI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]