Difference between revisions of "Part:BBa K5396004"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K5396004 short</partinfo>
 
<partinfo>BBa_K5396004 short</partinfo>
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<p>
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<p>This BARBIE1 protein is modified with an additional amino acid (cysteine). This enhancement allows it to be effectively utilized in our biosensor technology.</p>
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BARBIE1 is a synthetic protein derived from BaCBM2 (BBa_K5396003) through a process of reverse engineering. It has the increased ability to bind to plastics when compared to BaCBM2.</p><p>This BARBIE1 protein is modified with an additional amino acid (cysteine). This enhancement allows it to be effectively utilized in our biosensor technology.</p>
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===Usage and Biology===
 
===Usage and Biology===
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<p>
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BARBIE1 is a synthetic protein derived from BaCBM2 through a process of reverse engineering. It has the increased ability to bind to plastics when compared to BaCBM2.</p>
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===Part Generation===
 
<p>The BARBIE1-Cys fragment was generated from a PCR reaction using primers that specifically amplify the linker-BARBIE1-linker region of BBa_K5396001. The reverse primer used in this reaction adds a codon that encodes the amino acid cysteine at the end of the sequence.</p>
 
<p>The BARBIE1-Cys fragment was generated from a PCR reaction using primers that specifically amplify the linker-BARBIE1-linker region of BBa_K5396001. The reverse primer used in this reaction adds a codon that encodes the amino acid cysteine at the end of the sequence.</p>
  

Revision as of 11:34, 20 September 2024


Barbie1-Cys

This BARBIE1 protein is modified with an additional amino acid (cysteine). This enhancement allows it to be effectively utilized in our biosensor technology.

Usage and Biology

BARBIE1 is a synthetic protein derived from BaCBM2 through a process of reverse engineering. It has the increased ability to bind to plastics when compared to BaCBM2.

Part Generation

The BARBIE1-Cys fragment was generated from a PCR reaction using primers that specifically amplify the linker-BARBIE1-linker region of BBa_K5396001. The reverse primer used in this reaction adds a codon that encodes the amino acid cysteine at the end of the sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]