Difference between revisions of "Part:BBa K5321000&action=edit&section=1"

(Created page with "===Usage and Biology=== Spinach 2.1 RNA can bind and activate the fluorophore [http://pubs.acs.org/doi/abs/10.1021/ja410819x DFHBI-1T], both in vivo and in vitro. RNA molecul...")
 
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===Usage and Biology===
 
  
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Spinach 2.1 RNA can bind and activate the fluorophore [http://pubs.acs.org/doi/abs/10.1021/ja410819x DFHBI-1T], both in vivo and in vitro. RNA molecules can thus be fluorescently tagged, by gene fusion with this BioBrick.
 
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The flanking tRNA comes from human tRNALys3 and increases stability and folding efficiency of the RNA, leading to higher fluorescent signal.
 
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It is possible to detect signal from Spinach2.1 when expressed from moderate strength promoters, e.g. the [[Promoters/Catalog/Anderson|Anderson promoter library]].
 
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This part does not contain or need a Ribosome Binding Site.
 

Latest revision as of 11:12, 20 September 2024