Difference between revisions of "Part:BBa K216008:Design"
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===Source=== | ===Source=== | ||
| − | Amplified from genomic DNA of ''Xenorhabdus luminescens''. | + | Amplified from genomic DNA of ''Xenorhabdus luminescens'' strain Hw. |
===References=== | ===References=== | ||
Revision as of 14:39, 21 October 2009
bacterial luciferase LuxAB of Xenorhabdus luminescens
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 530
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1049
Design Notes
No special considerations. Please note that preliminary sequencing suggests that there may be a problem with the SpeI site of the construct submitted. If this is confirmed, we will submit a revised version and remove this note.
Source
Amplified from genomic DNA of Xenorhabdus luminescens strain Hw.
References
- Xi, L., Cho, K.W., and Tu, S.C. 1991. Cloning and nucleotide sequences of lux genes and characterization of luciferase of Xenorhabdus luminescens from a human wound. Journal of Bacteriology 173, 1399-1405.
- Westerlund-Karlsson, A., Saviranta, P., and Karp, M. 2002. Generation of thermostable monomeric luciferases from Photorhabdus luminescens. Biochemical and Biophysical Research Communications 296, 1072-1076.
- Mitchell, R.J., Ahn, J.M., and Gu, M.B. 2005. Comparison of Photorhabdus luminescens and Vibrio fischeri lux fusions to study gene expression patterns. Journal of Microbiology and Biotechnology 15, 48-54.