Difference between revisions of "Part:BBa K5492731"
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<partinfo>BBa_K5492731 short</partinfo>
 
<partinfo>BBa_K5492731 short</partinfo>
  
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Connects to BBa_K5492721 (H1_JOHNSON) (if histamine is not present)
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ssDNA sequence designed to be complementary for the 5' end of [https://parts.igem.org/Part:BBa_K5492721 j1_aptamer_with_flurophore], it contains a quencher molecule which absorbs the light emitted by flurophore at its 3' end, until the aptamer binds histamine.
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quencher /3iabrqsp/ - doesent allow for fluorescence when histamine isnt attached to BBa_K5492721 (H1_JOHNSON)
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===Usage and Biology===
 
===Usage and Biology===
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Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.
  
 
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Revision as of 10:30, 19 September 2024


J1_aptamer_reverse_complement_strand

ssDNA sequence designed to be complementary for the 5' end of j1_aptamer_with_flurophore, it contains a quencher molecule which absorbs the light emitted by flurophore at its 3' end, until the aptamer binds histamine.


Usage and Biology

Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]