Difference between revisions of "Part:BBa K5492720"
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[https://parts.igem.org/Part:BBa_K5492710 Y1 ssDNA aptamer] sequence designed to specifically bind to histamine. | [https://parts.igem.org/Part:BBa_K5492710 Y1 ssDNA aptamer] sequence designed to specifically bind to histamine. | ||
The sequence contains a fluorophore molecule at its 5' end. | The sequence contains a fluorophore molecule at its 5' end. | ||
| − | [https://parts.igem.org/Part:BBa_K5492730 y1_reverse_compliment_strand] is complementary to this sequnce, which | + | [https://parts.igem.org/Part:BBa_K5492730 y1_reverse_compliment_strand] is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by flurophore until the aptamer binds histamine. |
Revision as of 10:25, 19 September 2024
Y1_aptamer_with_fluorophore
Y1 ssDNA aptamer sequence designed to specifically bind to histamine. The sequence contains a fluorophore molecule at its 5' end. y1_reverse_compliment_strand is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by flurophore until the aptamer binds histamine.
Usage and Biology
Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]