Difference between revisions of "Part:BBa K5492721"

 
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<partinfo>BBa_K5492721 short</partinfo>
 
<partinfo>BBa_K5492721 short</partinfo>
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[https://parts.igem.org/Part:BBa_K5492711 J1 ssDNA aptamer] sequence designed to specifically bind to histamine.
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The sequence contains a fluorophore molecule at its 5' end.
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[https://parts.igem.org/Part:BBa_K5492731 j1_reverse_compliment_strand] is complementary to this sequnce, which acts as a quencher to flurophore until the aptamer binds histamine.
  
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Binds to histamine
 
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Connects to BBa_K5492731 (H1_JOHNSON_REVCOMP)
 
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fluorophor /5cy5/ is attached to the start of the sequence for when histamine is attached the fluorescence isnt blocked
 
  
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===Usage and Biology===
 
===Usage and Biology===
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Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.
  
 
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Revision as of 10:14, 19 September 2024


J1_aptamer_with_fluorophore J1 ssDNA aptamer sequence designed to specifically bind to histamine. The sequence contains a fluorophore molecule at its 5' end. j1_reverse_compliment_strand is complementary to this sequnce, which acts as a quencher to flurophore until the aptamer binds histamine.


Usage and Biology

Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]