Difference between revisions of "Part:BBa K191008:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Site specific mutation on plasmid with part <partinfo>BBa_K191003</partinfo>. | + | Site specific mutation (a.a#427 ILE -> PHE) on plasmid with part <partinfo>BBa_K191003</partinfo>. |
===Source=== | ===Source=== | ||
Revision as of 14:20, 21 October 2009
Promoter LacI - RBS - LovTAP(ILE427PHE) - Term
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 370
Illegal PstI site found at 627 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 370
Illegal PstI site found at 627 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 370
Illegal PstI site found at 627 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 370
Illegal PstI site found at 627 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 764
Design Notes
Site specific mutation (a.a#427 ILE -> PHE) on plasmid with part BBa_K191003.
Source
Mutagenesis kit of Stratagene was used.