Difference between revisions of "Part:BBa K5492701"

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<partinfo>BBa_K5492701 short</partinfo>
 
<partinfo>BBa_K5492701 short</partinfo>
  
J1 ssDNA sequence designed for serving as connector between avidin-coated magnetic beads and the 5' overhang of [https://parts.igem.org/Part:BBa_K5492711 J1_aptamer].  
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J1 ssDNA sequence designed for serving as connector between avidin-coated magnetic beads and the 5' overhang of [https://parts.igem.org/Part:BBa_K5492711 J1_aptamer] and [https://parts.igem.org/Part:BBa_K5492710 Y1_aptamer].  
 
The connector sequence contains a 5' biotin tag that lets the sequence bind to the bead via avidin-biotin connection.
 
The connector sequence contains a 5' biotin tag that lets the sequence bind to the bead via avidin-biotin connection.
  

Revision as of 09:41, 19 September 2024


J1_Y1_aptamer_connector

J1 ssDNA sequence designed for serving as connector between avidin-coated magnetic beads and the 5' overhang of J1_aptamer and Y1_aptamer. The connector sequence contains a 5' biotin tag that lets the sequence bind to the bead via avidin-biotin connection.


Usage and Biology

Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]