Difference between revisions of "Part:BBa K257010:Design"
(→Design Notes) |
(→Source) |
||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | + | Assembly of BBa_K257002 and BBa_K257008. | |
===References=== | ===References=== | ||
Revision as of 14:16, 21 October 2009
clyA + RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 84
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1494
Illegal AgeI site found at 1606 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
To avoid frameshift between clyA fusion C Term and RFP fusion N Term we use respectively the fusion silver standard suffix RFC 23 (for clyA C term, the prefix being standard RFC 10) and the fusion silver standard prefix RFC 23 (for RFP N Term, the suffix being standard RFC 10)
Source
Assembly of BBa_K257002 and BBa_K257008.