Difference between revisions of "Part:BBa K5374010"

Revision as of 09:10, 19 September 2024

SPARC/OD-EGFP. A collagen-binding domain involved in tissue repair and bone mineralization, fused wi

SPARC, containing the Osteonectin Domain (OD), interacts with the extracellular matrix to promote collagen binding and matrix remodeling. The fusion with EGFP allows for the assessment of the domain’s influence on EGFP activity, providing insight into how it may affect downstream protein functionality. This system helps screen potential tags for cell factor release in tissue engineering.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 484
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 484
Illegal NheI site found at 922
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 484
Illegal BglII site found at 897
Illegal BamHI site found at 288
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 484
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 484
Illegal NgoMIV site found at 859
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1565

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5374010 short</partinfo>
-SPARC/OD was fused with EGFP to study the effect of this tag on subsequent connexin activity.
+SPARC, containing the Osteonectin Domain (OD), interacts with the extracellular matrix to promote collagen binding and matrix remodeling. The fusion with EGFP allows for the assessment of the domain’s influence on EGFP activity, providing insight into how it may affect downstream protein functionality. This system helps screen potential tags for cell factor release in tissue engineering.
 <!-- Add more about the biology of this part here