Difference between revisions of "Part:BBa K191008:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K191008 short</partinfo>
 
<partinfo>BBa_K191008 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Mutagenesis kit of Stratagene was used.  
+
Site specific mutation on plasmid with part <partinfo>BBa_K191003</partinfo>.
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+
−
 
+
  
 
===Source===
 
===Source===
 +
Mutagenesis kit of Stratagene was used.
  
−
Site specific mutation on plasmid with part BBa_K191003
 
  
 
===References===
 
===References===

Revision as of 14:09, 21 October 2009

Promoter LacI - RBS - LovTAP(ILE427PHE) - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 764


Design Notes

Site specific mutation on plasmid with part BBa_K191003.

Source

Mutagenesis kit of Stratagene was used.


References