Difference between revisions of "Part:BBa K201004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was designed with the same structure of the Berkley constitutive promoters. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. | + | This part was designed with the same structure of the [https://parts.igem.org/Part:BBa_J23100 Berkley constitutive promoters]. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. |
This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp). | This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp). | ||
Revision as of 13:59, 21 October 2009
Lac O2 natural operator with RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 582
Illegal AgeI site found at 694 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed with the same structure of the Berkley constitutive promoters. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp).
Source
All subparts come from the Registry of Standard Parts