Difference between revisions of "Part:BBa K243029:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br> | + | Glycine and serine are good suitable for repetitive sequences.The difference to our [https://parts.igem.org/Part:BBa_K243030 GSATLinker] is that the sequence produce some glutamic acid and alanin amino acids.The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br> |
[[Image:Freiburg09 Gsatpart.png|750x550px]]<br> | [[Image:Freiburg09 Gsatpart.png|750x550px]]<br> | ||
[https://static.igem.org/mediawiki/parts/a/a4/Freiburg09_GSAT.txt Commented GenBank file] | [https://static.igem.org/mediawiki/parts/a/a4/Freiburg09_GSAT.txt Commented GenBank file] | ||
Revision as of 13:59, 21 October 2009
GSAT Linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Glycine and serine are good suitable for repetitive sequences.The difference to our GSATLinker is that the sequence produce some glutamic acid and alanin amino acids.The properties zwitterionic and hydrophile prevent the aggregation of the linker.
The sequence has no cleavage site for proteases.
Commented GenBank file
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Source
Planed and designed by Team Freiburg Bioware and synthesized by Mr.Gene.