Difference between revisions of "Part:BBa K5043011:Design"

 
m (→‎Source)
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Acropora millepora. Protein coding sequence was taken from iGEM 2024 distribution kit, regulatory elements were ordered from IDT.
+
Derived from Acropora millepora. [9] Protein coding sequence was taken from iGEM 2024 distribution kit, regulatory elements were ordered from IDT.
  
 
===References===
 
===References===

Latest revision as of 12:46, 18 September 2024


amilGFP transcriptional unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 161
    Illegal AgeI site found at 622
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

amilGFP was taken from iGEM 2024 distribution kit. RBS and restriction sides for BsaI were added using overhang-PCR to enable GoldenGate assembly.


Source

Derived from Acropora millepora. [9] Protein coding sequence was taken from iGEM 2024 distribution kit, regulatory elements were ordered from IDT.

References