Difference between revisions of "Part:BBa K5492403"

Revision as of 20:23, 17 September 2024

Optimised HNMT - pETDUET-1

Optimised coding sequence of human Histamine-N-Methyltransferase appropiated for insertion into pETDUET-1.

Usage and Biology

Histamine-N-Methlytransferase (HNMT) takes part in the inactivation of histamine. It transfers a methyl group to histamine, and the resulting N-methyl histamine isn’t able to bind to histamine receptors, thus reducing the biological effect of the compound. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal BglII site found at 1015
Illegal BamHI site found at 123
Illegal XhoI site found at 1064
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47
Illegal NgoMIV site found at 1034
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Optimised coding sequence of human Histamine-N-Methyltransferase appropiated for insertion into [https://parts.igem.org/Part:BBa_K5492101 pETDUET-1].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Histamine-N-Methlytransferase (HNMT) takes part in the inactivation of histamine. It transfers a methyl group to histamine, and the resulting N-methyl histamine isn’t able to bind to histamine receptors, thus reducing the biological effect of the compound. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.
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