Difference between revisions of "Part:BBa K5492203"

Revision as of 20:16, 17 September 2024

Optimised DAO - pETDUET-1

Optimised coding sequence of rat Diamine Oxidase appropiated for insertion into pETDUET-1.

Usage and Biology

Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine, and the resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 129
Illegal PstI site found at 190
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 129
Illegal BglII site found at 2392
Illegal BamHI site found at 123
Illegal BamHI site found at 2067
Illegal BamHI site found at 2321
Illegal XhoI site found at 937
Illegal XhoI site found at 2441
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190
Illegal NgoMIV site found at 2411
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Optimised coding sequence of rat Diamine Oxidase appropiated for insertion into [https://parts.igem.org/Part:BBa_K5492101 pETDUET-1].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine, and the resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.
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