Difference between revisions of "Part:BBa K216005:Experience"
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Edinburgh iGEM team, October 2009: To test this part, it was combined with lacZ minigene J33202 for Miller assays (giving part BBa_K216009), and also with GFP for analysis using the promoter characterization kit of Jason Kelly. Results from overnight induction with Miller assay indicated stronger induction by nitrite than nitrate, but a time course of induction using the GFP construct indicated that nitrate gave stronger induction in the first few hours. | Edinburgh iGEM team, October 2009: To test this part, it was combined with lacZ minigene J33202 for Miller assays (giving part BBa_K216009), and also with GFP for analysis using the promoter characterization kit of Jason Kelly. Results from overnight induction with Miller assay indicated stronger induction by nitrite than nitrate, but a time course of induction using the GFP construct indicated that nitrate gave stronger induction in the first few hours. | ||
<p> | <p> | ||
| − | Results from Miller's Assay | + | <b>Results from Miller's Assay</b> |
<br> | <br> | ||
[[Image:PyeaR-miller.png]] | [[Image:PyeaR-miller.png]] | ||
<br> | <br> | ||
| − | Results from GFP promoter characterization kit. | + | <b>Results from GFP promoter characterization kit.</b> |
<br> | <br> | ||
[[Image:Pyear_characterization.jpg]] | [[Image:Pyear_characterization.jpg]] | ||
Revision as of 13:41, 21 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K216005
Edinburgh iGEM team, October 2009: To test this part, it was combined with lacZ minigene J33202 for Miller assays (giving part BBa_K216009), and also with GFP for analysis using the promoter characterization kit of Jason Kelly. Results from overnight induction with Miller assay indicated stronger induction by nitrite than nitrate, but a time course of induction using the GFP construct indicated that nitrate gave stronger induction in the first few hours.
Results from Miller's Assay
Results from GFP promoter characterization kit.
User Reviews
UNIQb4d050381c8ff663-partinfo-00000000-QINU
UNIQb4d050381c8ff663-partinfo-00000001-QINU