Difference between revisions of "Part:BBa K5398030"

 
m
Line 4: Line 4:
  
 
In the Mfp5-TRn4 fusion proteins, Mfp5 is derived from the mussel foot proteins, whose tyrosine residues are oxidized by tyrosinase into dopamine, which primarily forms π-π bonds and hydrogen bonds with the surface materials, allowing them to adhere to various materials. TRn4 is a protein obtained by repeating the sequence from squid ring teeth proteins four times, and the β-sheet on its structure can connect with the β-sheet on the structure of highly repetitive squid ring teeth proteins through hydrogen bonds. Tyrosine on Mfp5 generates dopa when tyrosinase is present, which makes Mfp5-TRn4 fusion protein adhere to the surface materials.  
 
In the Mfp5-TRn4 fusion proteins, Mfp5 is derived from the mussel foot proteins, whose tyrosine residues are oxidized by tyrosinase into dopamine, which primarily forms π-π bonds and hydrogen bonds with the surface materials, allowing them to adhere to various materials. TRn4 is a protein obtained by repeating the sequence from squid ring teeth proteins four times, and the β-sheet on its structure can connect with the β-sheet on the structure of highly repetitive squid ring teeth proteins through hydrogen bonds. Tyrosine on Mfp5 generates dopa when tyrosinase is present, which makes Mfp5-TRn4 fusion protein adhere to the surface materials.  
 +
 +
<html>
 +
<center><img src="https://static.igem.wiki/teams/5398/trn4-mfp5/pet-pc-sumo-8-8-map.webp"with="1000" height="" width="500" height=""/></center>
 +
</html>
 +
 +
<p style="text-align: center!important;"><b>Fig. 1 The plasmid map of TRn4-mfp5.
 +
</b></p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:10, 17 September 2024


A fusion protein that adheres to the surface of dentate ring protein and substrate of squid.

In the Mfp5-TRn4 fusion proteins, Mfp5 is derived from the mussel foot proteins, whose tyrosine residues are oxidized by tyrosinase into dopamine, which primarily forms π-π bonds and hydrogen bonds with the surface materials, allowing them to adhere to various materials. TRn4 is a protein obtained by repeating the sequence from squid ring teeth proteins four times, and the β-sheet on its structure can connect with the β-sheet on the structure of highly repetitive squid ring teeth proteins through hydrogen bonds. Tyrosine on Mfp5 generates dopa when tyrosinase is present, which makes Mfp5-TRn4 fusion protein adhere to the surface materials.

Fig. 1 The plasmid map of TRn4-mfp5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1035
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 189
    Illegal XhoI site found at 1018
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]