This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our FluA and DigA domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly .
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[edit] Usage and Biology
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Linker were used normally to connect two peptides, it is important for that connection,that the linker itself has no influence of the connected peptides. So the sequence of the linker is designed for amino acids which not interact with the environment. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.
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The length of the linker is important to guarantee the independent function of two connected parts. When the linker is too short there might be a sterical interference between the parts and when it is too long, it can cause an instability of the construct.Also it is important that the linker has a certain flexibility.
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Revision as of 13:31, 21 October 2009
Strep-FluA-Middle Linker-Fok_a
This combination of parts use the benefits of an Strep-tag for purification. It is also linked with a FluroescineA tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee
the independent function of FluA tag and the protein domain Fok_a.
