Difference between revisions of "Part:BBa K243030"
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<partinfo>BBa_K243030 short</partinfo> | <partinfo>BBa_K243030 short</partinfo> | ||
| − | This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The | + | This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The SEG linker was create to connect our protein domains [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243000 Fok_a] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243001 Fok_i] to a final construct. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:25, 21 October 2009
SEG
This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The SEG linker was create to connect our protein domains Fok_a and Fok_i to a final construct.
Usage and Biology
Linker were used normally to connect two peptides, it is important for that connection,that the linker itself has no influence of the connected peptides. So the sequence of the linker is designed for amino acids which not interact with the environment. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.
The length of the linker is important to guarantee the independent function of two connected parts. When the linker is too short there might be a sterical interference between the parts and when it is too long, it can cause an instability of the construct.Also it is important that the linker has a certain flexibility.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]