Difference between revisions of "Part:BBa K243006:Design"
(→Source) |
(→Design Notes) |
||
| Line 8: | Line 8: | ||
Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br> | Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br> | ||
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.<br> | Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.<br> | ||
| − | + | [[Image:Freiburg09 Longlipart.png|750x550px]]<br> | |
[https://static.igem.org/mediawiki/parts/8/84/LongLinker.txt Commented GenBank file] | [https://static.igem.org/mediawiki/parts/8/84/LongLinker.txt Commented GenBank file] | ||
Revision as of 13:15, 21 October 2009
Long Linker (Gly-Gly-Ser-Gly)x3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.
The sequence has no cleavage site for proteases.
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.
Commented GenBank file
Source
Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR.