Difference between revisions of "Part:BBa K5317001"

Revision as of 07:22, 16 September 2024

mRuby2

Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K5317001 short</partinfo>
-PknB is a eukaryote-like serine/threonine kinase in Staphylococcus aureus that consists of an N-terminal cytosolic kinase domain, a central transmembrane domain and three C-terminal extracellular PASTA domains.2 When this extracellular domain recognises beta-lactam compounds, it transmits the signal by autophosphorylating the N-terminal kinase domain, thereby activating downstream signalling cascades.3 In S. aureus, this mechanism normally enables early recognition and adaptation to antibiotic stress.4 In our project, we aim to introduce PknB into HEK cells, together with three proteins that can be phosphorylated by PknB. This targeted phosphorylation increases DNA binding activity 1 and is expected to induce the expression of a specific gene, which generates a fluorescent signal.
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