Difference between revisions of "Part:BBa K176248"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176248 short</partinfo>
 
<partinfo>BBa_K176248 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K176248 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K176248 SequenceAndFeatures</partinfo>
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===Model===
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To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-11 here] please.
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Latest revision as of 13:09, 21 October 2009

Low input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229

PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)

Cell Death = (Cell Density * (pCon 0.15 + Population Average (1 / (1 + Input PoPS))) + Input AHL) * Input PoPS

Cells with relative low input score in the population will be selected, cells with relative high input score will die.

The first luxI-LVA is used to calculate average score in the population.

mRFP is input score reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3251
    Illegal NheI site found at 3274
    Illegal NheI site found at 4100
    Illegal NheI site found at 4123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2382
    Illegal BglII site found at 3942
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5567
    Illegal BsaI.rc site found at 5078


Model

To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-11 here] please.