Difference between revisions of "Part:BBa K243005:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 9: Line 9:
 
The sequence has no cleavage site for proteases. <br>
 
The sequence has no cleavage site for proteases. <br>
 
[[Image:Freiburg09 Middlelipart.png|750x550px]] <br>
 
[[Image:Freiburg09 Middlelipart.png|750x550px]] <br>
−
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.<br>
+
Designed for the fusion of proteins or peptides via in frame cloning, according to [https://parts.igem.org/Assembly_standard_25 RFC 25].<br>
−
 
+
 
[https://static.igem.org/mediawiki/parts/b/bd/MiddleLinker.txt Commented GenBank file]
 
[https://static.igem.org/mediawiki/parts/b/bd/MiddleLinker.txt Commented GenBank file]
  

Revision as of 13:08, 21 October 2009

Middle Linker ( Gly-Gly-Ser-Gly)x2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.
The sequence has no cleavage site for proteases.
Freiburg09 Middlelipart.png
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Commented GenBank file

Source

Oligos synthesized by sigma. Hybridized by PCR.

References