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    <title>Protein Description</title>
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ScCBD_cex'V1 : a Cellulose Binding Domain optimised for Saccharomyces cerevisiae, that enables the fixation of proteins to Bacterial Cellulose
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    <h1>Description</h1>
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    <p>
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        ScCBD_cex'V1 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V1 enables the fixation of the protein on the bacterial cellulose.
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-1.png" width="200" alt="CBD_CexV1">
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            <figcaption>Figure 1: ScCBD_Cex'V1 three-dimensional structure</figcaption>
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        <figure>
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-2.png" width="400" alt="Functional cellulose">
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            <figcaption>Figure 2: Functional cellulose</figcaption>
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        </figure>
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        </figure>
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-3.png" width="100" alt="ScCBD_Cex coding sequence">
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            <figcaption>Figure 3: ScCBD_Cex coding sequence</figcaption>
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    <h1>Construction</h1>
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    <p>
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      ScCBD-Cex'V1 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a yellow chromoprotein (fwYellow) in order to color the bacterial cellulose in yellow: <a href="https://parts.igem.org/Part:BBa_K5143023" target="_blank">BBa_K5143023</a>
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    <h1>References</h1>
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    <p>
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        Cappellaro, C. et al. Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction. The EMBO Journal 10, 4081–4088 (1991)
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
===Functional Parameters===
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Revision as of 12:40, 15 September 2024


AGA2, a pre-peptide that is secreted by the Saccharomyces cerevisiae yeast

Protein Description ScCBD_cex'V1 : a Cellulose Binding Domain optimised for Saccharomyces cerevisiae, that enables the fixation of proteins to Bacterial Cellulose

Description

ScCBD_cex'V1 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V1 enables the fixation of the protein on the bacterial cellulose.

CBD_CexV1
Figure 1: ScCBD_Cex'V1 three-dimensional structure
Functional cellulose
Figure 2: Functional cellulose
ScCBD_Cex coding sequence
Figure 3: ScCBD_Cex coding sequence

Construction

ScCBD-Cex'V1 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a yellow chromoprotein (fwYellow) in order to color the bacterial cellulose in yellow: BBa_K5143023

References

Cappellaro, C. et al. Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction. The EMBO Journal 10, 4081–4088 (1991)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]