Difference between revisions of "Part:BBa K5143008"
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     <title>Protein Description</title>
 
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ScCBD_cex'V1 : a Cellulose Binding Domain optimised for Saccharomyces cerevisiae, that enables the fixation of proteins to Bacterial Cellulose
 
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     <h1>Description</h1>
 
     <h1>Description</h1>
 
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     <p>
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         ECRIRE DES TRUCS ICI POUR LA DESCRIPTION
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         ScCBD_cex'V2 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V2 enables the fixation of the protein on the bacterial cellulose. This version of ScCBD_Cex has been modified to avoid recombination with ScCBD_Cex'V1 in the yeast.
 
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-1.png" width="200" alt="CBD_CexV2">
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            <figcaption>Figure 1: ScCBD_Cex'V2 three-dimensional structure</figcaption>
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        </figure>
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        <figure>
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-2.png" width="400" alt="Functional cellulose">
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            <figcaption>Figure 2: Functional cellulose</figcaption>
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-3.png" width="100" alt="ScCBD_Cex coding sequence">
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            <figcaption>Figure 3: ScCBD_Cex coding sequence</figcaption>
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     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
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       ScCBD-Cex'V2 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This optimised sequence has been modified to avoid recombination with ScCBD-Cex'V1 in the Yeast. ScCDB-Cex'V2 protein is used in fusion with bioglue composite biobrick (Cp19k_MaSp1) in order to functionalise bacterial cellulose with sticky properties: <a href="https://parts.igem.org/Part:BBa_K5143022" target="_blank">BBa_K5143022</a>
 
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     <h1>References</h1>
 
     <h1>References</h1>
 
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         METTRE LES REF ICI
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         Ong, E., Gilkes, N. R., Miller, R. C., Warren, R. A. & Kilburn, D. G. Te
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cellulose-binding domain (CBD(Cex)) of an exoglucanase from Cellulomonas
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fmi: production in Escherichia coli and characterization of the polypeptide.
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Biotechnol. Bioeng. 42, 401–409 (1993).
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Gilbert, C.; Tang, T.-C.; Ott, W.; Dorr, B. A.; Shaw, W. M.; Sun, G. L.; Lu, T. K.; Ellis, T. Living Materials with Programmable Functionalities Grown from Engineered Microbial Co-Cultures. Nat. Mater. 2021, 20 (5), 691–700. https://doi.org/10.1038/s41563-020-00857-5.
 
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K5143007 parameters</partinfo>
 
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Revision as of 11:43, 15 September 2024


ScCBD_cex'V2 : a Cellulose Binding Domain, that enables the fixation of proteins to Cellulose


Protein Description ScCBD_cex'V1 : a Cellulose Binding Domain optimised for Saccharomyces cerevisiae, that enables the fixation of proteins to Bacterial Cellulose

Description

ScCBD_cex'V2 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V2 enables the fixation of the protein on the bacterial cellulose. This version of ScCBD_Cex has been modified to avoid recombination with ScCBD_Cex'V1 in the yeast.

CBD_CexV2
Figure 1: ScCBD_Cex'V2 three-dimensional structure
Functional cellulose
Figure 2: Functional cellulose
ScCBD_Cex coding sequence
Figure 3: ScCBD_Cex coding sequence

Construction

ScCBD-Cex'V2 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This optimised sequence has been modified to avoid recombination with ScCBD-Cex'V1 in the Yeast. ScCDB-Cex'V2 protein is used in fusion with bioglue composite biobrick (Cp19k_MaSp1) in order to functionalise bacterial cellulose with sticky properties: BBa_K5143022

References

Ong, E., Gilkes, N. R., Miller, R. C., Warren, R. A. & Kilburn, D. G. Te cellulose-binding domain (CBD(Cex)) of an exoglucanase from Cellulomonas fmi: production in Escherichia coli and characterization of the polypeptide. Biotechnol. Bioeng. 42, 401–409 (1993). Gilbert, C.; Tang, T.-C.; Ott, W.; Dorr, B. A.; Shaw, W. M.; Sun, G. L.; Lu, T. K.; Ellis, T. Living Materials with Programmable Functionalities Grown from Engineered Microbial Co-Cultures. Nat. Mater. 2021, 20 (5), 691–700. https://doi.org/10.1038/s41563-020-00857-5.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]