Difference between revisions of "Part:BBa K5143007"
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     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
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       ScCBD-Cex_V1 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a yellow chromoprotein (fwYellow) in order to color the bacterial cellulose in yellow: <a href="https://parts.igem.org/Part:BBa_K5143023" target="_blank">BBa_K5143023</a> .
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       ScCBD-Cex_V1 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a yellow chromoprotein (fwYellow) in order to color the bacterial cellulose in yellow: <a href="https://parts.igem.org/Part:BBa_K5143023" target="_blank">BBa_K5143023</a>.
 
     </p>
 
     </p>
 
     <h1>References</h1>
 
     <h1>References</h1>

Revision as of 09:45, 15 September 2024

Protein Description ScCBD_cex'V1 : a Cellulose Binding Domain optimised for Saccharomyces cerevisiae, that enables the fixation of proteins to Bacterial Cellulose

Description

ScCBD_cex'V1 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V1 enables the fixation of the protein on the bacterial cellulose.

CBD_CexV1
Figure 1: ScCBD_CexV1 three-dimensional structure
Functional cellulose
Figure 2: Functional cellulose

Construction

ScCBD-Cex_V1 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a yellow chromoprotein (fwYellow) in order to color the bacterial cellulose in yellow: BBa_K5143023.

References

Ong, E., Gilkes, N. R., Miller, R. C., Warren, R. A. & Kilburn, D. G. Te cellulose-binding domain (CBD(Cex)) of an exoglucanase from Cellulomonas fmi: production in Escherichia coli and characterization of the polypeptide. Biotechnol. Bioeng. 42, 401–409 (1993). Gilbert, C.; Tang, T.-C.; Ott, W.; Dorr, B. A.; Shaw, W. M.; Sun, G. L.; Lu, T. K.; Ellis, T. Living Materials with Programmable Functionalities Grown from Engineered Microbial Co-Cultures. Nat. Mater. 2021, 20 (5), 691–700. https://doi.org/10.1038/s41563-020-00857-5.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]