Difference between revisions of "Part:BBa K5407000"
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pLKO.1 plasmid is a basic backbone plasmid and can be expressed in mammalian cell lines. The plasmid map can be viewed in the figure 1.The backbone size of the plasmid is 7032 bp,and the selectable markers of the plasmid is puromycin Scientists often use the plasmid tool to inhibit the expression of genes. In our study, we cloned the siRNA sequence targeted to TEAD4 into the basic part pLKO.1 plasmid. And we constructed two composite plasmid named sh-TEAD4-1 and sh-TEAD4-2.Then the TEAD4 mRNA could be degraded in the SW480 cell. | pLKO.1 plasmid is a basic backbone plasmid and can be expressed in mammalian cell lines. The plasmid map can be viewed in the figure 1.The backbone size of the plasmid is 7032 bp,and the selectable markers of the plasmid is puromycin Scientists often use the plasmid tool to inhibit the expression of genes. In our study, we cloned the siRNA sequence targeted to TEAD4 into the basic part pLKO.1 plasmid. And we constructed two composite plasmid named sh-TEAD4-1 and sh-TEAD4-2.Then the TEAD4 mRNA could be degraded in the SW480 cell. | ||
| − | + | https://static.igem.wiki/teams/5407/plko.png | |
Figure 1 pLKO.1 map | Figure 1 pLKO.1 map | ||
Revision as of 08:00, 15 September 2024
GalR
Usage and Biology
pLKO.1 plasmid is a basic backbone plasmid and can be expressed in mammalian cell lines. The plasmid map can be viewed in the figure 1.The backbone size of the plasmid is 7032 bp,and the selectable markers of the plasmid is puromycin Scientists often use the plasmid tool to inhibit the expression of genes. In our study, we cloned the siRNA sequence targeted to TEAD4 into the basic part pLKO.1 plasmid. And we constructed two composite plasmid named sh-TEAD4-1 and sh-TEAD4-2.Then the TEAD4 mRNA could be degraded in the SW480 cell.
Figure 1 pLKO.1 map
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1645
Illegal SpeI site found at 1182 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1645
Illegal SpeI site found at 1182
Illegal NotI site found at 1931
Illegal NotI site found at 2829 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1645
Illegal BglII site found at 107
Illegal BglII site found at 148
Illegal BglII site found at 214
Illegal BglII site found at 3501
Illegal BamHI site found at 925
Illegal XhoI site found at 1444
Illegal XhoI site found at 1634
Illegal XhoI site found at 1938 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1645
Illegal SpeI site found at 1182 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1645
Illegal SpeI site found at 1182
Illegal NgoMIV site found at 6296
Illegal AgeI site found at 1669 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 426
Illegal BsaI.rc site found at 125
Illegal BsaI.rc site found at 1340
Illegal BsaI.rc site found at 3519
Illegal BsaI.rc site found at 5120
Illegal SapI site found at 2866
Illegal SapI site found at 4037