Difference between revisions of "Part:BBa K243027"
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Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. One of them is the so called c-Fos. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).  
 
Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. One of them is the so called c-Fos. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).  
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The dimerisation of the molecules generates the AP-1 transcription factor for upregulation of several different genes.
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The dimerisation of the molecules of the Fos family with c-Jun generates the AP-1 transcription factor for upregulation of several different genes.
  
  

Revision as of 12:53, 21 October 2009

His-FOS

Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. One of them is the so called c-Fos. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July). The dimerisation of the molecules of the Fos family with c-Jun generates the AP-1 transcription factor for upregulation of several different genes.



1fos bio r 250.jpg Heterodimeric Fos complex (http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS)


Usage and Biology

Plasmids encoding the sequences for the bZip domains of c-Fos linked to a His-tag allow the purification which could then be expressed as soluble proteins in Escherichia coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]