Difference between revisions of "Part:BBa K243028"

Revision as of 12:48, 21 October 2009

pMA FOS-Split Linker-Fok_a

Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. One of them is the so called c-Fos. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July). The dimerisation of the molecules generates the AP-1 transcription factor for upregulation of several different genes.

Heterodimeric Fos complex (http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS)

Usage and Biology

Split Linker-Fok_a can be fused with Fos. The mixture of this construct with an other hybrid protein GST-FosW-His (simultanously expressed) allows the dimerization of Fos and therefore its binding at their target sequence TRE (TGACTCA) or CRE (TGACGTCA).

@@ Line 1: / Line 1: @@
 == pMA FOS-Split Linker-Fok_a ==
-Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).
+Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. One of them is the so called c-Fos. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July). The dimerisation of the molecules generates the AP-1 transcription factor for upregulation of several different genes.
 [[Image:1fos bio r 250.jpg]]Heterodimeric Fos complex (http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS)