Difference between revisions of "Part:BBa K5317008:Design"

 
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===Design Notes===
 
===Design Notes===
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It was important to consider the MRE site positioning for optimal MTF-1 and MRE site interaction and do not diturb the start and stop codon as well as avoid frame shifts of the EGFP sequence during cloning.  
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It was important to consider the MRE site positioning for optimal MTF-1 and MRE site interaction and not disturb the start and stop codon as well as avoid frame shifts of the EGFP sequence during cloning.  
  
  

Revision as of 19:00, 14 September 2024


MREwt promoter-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was important to consider the MRE site positioning for optimal MTF-1 and MRE site interaction and not disturb the start and stop codon as well as avoid frame shifts of the EGFP sequence during cloning.


Source

The MRE wt promoter was synthesized and the EGFP gene was available from the EGFP-C2 backbone (K3338020)

References