Difference between revisions of "Part:BBa K180010:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K180010 short</partinfo>
 
<partinfo>BBa_K180010 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).
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BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.
  
  

Latest revision as of 12:42, 21 October 2009

Rock-paper-scissors - Rock primary plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 893
    Illegal NheI site found at 916
    Illegal PstI site found at 2760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 622
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2760
    Illegal AgeI site found at 3365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 147


Design Notes

BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.


Source

See relevant biobrick component parts (BBa_K180011 and BBa_K180012).

References