Difference between revisions of "Part:BBa K5226018"

 
 
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<partinfo>BBa_K5226018 short</partinfo>
 
<partinfo>BBa_K5226018 short</partinfo>
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===Sequence and Features===
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<partinfo>BBa_K5226018 SequenceAndFeatures</partinfo>
  
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Lac Operator sequence that is bound by LacI repressor. When attached downstream of a promoter this will inhibit transcription of any genes downstream. Therefore, this part can be used to create a repressed promoter belonging to any class.
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===Introduction===
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In order to reduce the leakage level of the Mmp1 promoter, one more lacO was introduced immediately after the transcription start site (+1) of PT7-like (producing PT7-like-LacO).The Mmp1 inducible promoter currently used by our team has undergone various optimizations as described above.
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Li et al. [1] analyzed the proteomic characteristics of <i>Halomonas</i> TD01 using SDS-PAGE. They identified <B>a strongly expressed endogenous gene encoding a porin and preliminarily determined its promoter region</B>. Furthermore, they <b>identified the core region and constructed a constitutive promoter library by randomizing the sequences between the -35 and -10 regions</b>. Shen et al. [2] <b>performed 3-nucleotide saturation mutagenesis upstream of the -10 box and 4-nucleotide saturation mutagenesis within the -10 box to further expand the promoter library</b>.
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We have only included the porin constitutive promoters that are relevant to our team.
  
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<!-- Add more about the biology of this part here
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===References===
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===Usage and Biology===
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[1]Li T, Li T, Ji W, et al. Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp[J]. Biotechnology Journal, 2016, 11(2): 219-227.
  
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[2]Shen R, Yin J, Ye JW, Xiang RJ, Ning ZY, Huang WZ, Chen GQ. Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis. ACS Synth Biol. 2018 Aug 17;7(8):1897-1906. doi: 10.1021/acssynbio.8b00102. Epub 2018 Jul 31. PMID: 30024739.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5226018 SequenceAndFeatures</partinfo>
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Latest revision as of 05:15, 14 September 2024


porin278

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

Li et al. [1] analyzed the proteomic characteristics of Halomonas TD01 using SDS-PAGE. They identified a strongly expressed endogenous gene encoding a porin and preliminarily determined its promoter region. Furthermore, they identified the core region and constructed a constitutive promoter library by randomizing the sequences between the -35 and -10 regions. Shen et al. [2] performed 3-nucleotide saturation mutagenesis upstream of the -10 box and 4-nucleotide saturation mutagenesis within the -10 box to further expand the promoter library. We have only included the porin constitutive promoters that are relevant to our team.

References

[1]Li T, Li T, Ji W, et al. Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp[J]. Biotechnology Journal, 2016, 11(2): 219-227.

[2]Shen R, Yin J, Ye JW, Xiang RJ, Ning ZY, Huang WZ, Chen GQ. Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis. ACS Synth Biol. 2018 Aug 17;7(8):1897-1906. doi: 10.1021/acssynbio.8b00102. Epub 2018 Jul 31. PMID: 30024739.