Difference between revisions of "Part:BBa K5226029"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K5226029 short</partinfo>
 
<partinfo>BBa_K5226029 short</partinfo>
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===Sequence and Features===
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<partinfo>BBa_K5226029 SequenceAndFeatures</partinfo>
  
 
===Introduction===
 
===Introduction===
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Li et al. [1] analyzed the proteomic characteristics of Halomonas TD01 using SDS-PAGE. They identified a strongly expressed endogenous gene encoding a porin and preliminarily determined its promoter region. Furthermore, they identified the core region and constructed a constitutive promoter library by randomizing the sequences between the -35 and -10 regions. Shen et al. [2] performed 3-nucleotide saturation mutagenesis upstream of the -10 box and 4-nucleotide saturation mutagenesis within the -10 box to further expand the promoter library.
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Li et al. [1] analyzed the proteomic characteristics of <i>Halomonas</i> TD01 using SDS-PAGE. They identified <B>a strongly expressed endogenous gene encoding a porin and preliminarily determined its promoter region</B>. Furthermore, they <b>identified the core region and constructed a constitutive promoter library by randomizing the sequences between the -35 and -10 regions</b>. Shen et al. [2] <b>performed 3-nucleotide saturation mutagenesis upstream of the -10 box and 4-nucleotide saturation mutagenesis within the -10 box to further expand the promoter library</b>.
 
We have only included the porin constitutive promoters that are relevant to our team.
 
We have only included the porin constitutive promoters that are relevant to our team.
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===Sequence and Features===
 
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<partinfo>BBa_K5226029 SequenceAndFeatures</partinfo>
 
  
 
===References===
 
===References===
 
[1]Li T, Li T, Ji W, et al. Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp[J]. Biotechnology Journal, 2016, 11(2): 219-227.
 
[1]Li T, Li T, Ji W, et al. Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp[J]. Biotechnology Journal, 2016, 11(2): 219-227.
  
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[2]Shen R, Yin J, Ye JW, Xiang RJ, Ning ZY, Huang WZ, Chen GQ. Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis. ACS Synth Biol. 2018 Aug 17;7(8):1897-1906. doi: 10.1021/acssynbio.8b00102. Epub 2018 Jul 31. PMID: 30024739.
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 +
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===References===
 +
[1]Li T, Li T, Ji W, et al. Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp[J]. Biotechnology Journal, 2016, 11(2): 219-227.
 +
<br>
 
[2]Shen R, Yin J, Ye JW, Xiang RJ, Ning ZY, Huang WZ, Chen GQ. Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis. ACS Synth Biol. 2018 Aug 17;7(8):1897-1906. doi: 10.1021/acssynbio.8b00102. Epub 2018 Jul 31. PMID: 30024739.
 
[2]Shen R, Yin J, Ye JW, Xiang RJ, Ning ZY, Huang WZ, Chen GQ. Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis. ACS Synth Biol. 2018 Aug 17;7(8):1897-1906. doi: 10.1021/acssynbio.8b00102. Epub 2018 Jul 31. PMID: 30024739.
  

Revision as of 03:03, 14 September 2024


porin281

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

Li et al. [1] analyzed the proteomic characteristics of Halomonas TD01 using SDS-PAGE. They identified a strongly expressed endogenous gene encoding a porin and preliminarily determined its promoter region. Furthermore, they identified the core region and constructed a constitutive promoter library by randomizing the sequences between the -35 and -10 regions. Shen et al. [2] performed 3-nucleotide saturation mutagenesis upstream of the -10 box and 4-nucleotide saturation mutagenesis within the -10 box to further expand the promoter library. We have only included the porin constitutive promoters that are relevant to our team.

References

[1]Li T, Li T, Ji W, et al. Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp[J]. Biotechnology Journal, 2016, 11(2): 219-227.

[2]Shen R, Yin J, Ye JW, Xiang RJ, Ning ZY, Huang WZ, Chen GQ. Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis. ACS Synth Biol. 2018 Aug 17;7(8):1897-1906. doi: 10.1021/acssynbio.8b00102. Epub 2018 Jul 31. PMID: 30024739.


References

[1]Li T, Li T, Ji W, et al. Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp[J]. Biotechnology Journal, 2016, 11(2): 219-227.
[2]Shen R, Yin J, Ye JW, Xiang RJ, Ning ZY, Huang WZ, Chen GQ. Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis. ACS Synth Biol. 2018 Aug 17;7(8):1897-1906. doi: 10.1021/acssynbio.8b00102. Epub 2018 Jul 31. PMID: 30024739.