Difference between revisions of "Part:BBa K5226028"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K5226028 short</partinfo> | <partinfo>BBa_K5226028 short</partinfo> | ||
| + | ===Sequence and Features=== | ||
| + | <partinfo>BBa_K5226028 SequenceAndFeatures</partinfo> | ||
===Introduction=== | ===Introduction=== | ||
| − | Li et al. [1] analyzed the proteomic characteristics of Halomonas TD01 using SDS-PAGE. They identified a strongly expressed endogenous gene encoding a porin and preliminarily determined its promoter region. Furthermore, they identified the core region and constructed a constitutive promoter library by randomizing the sequences between the -35 and -10 regions. Shen et al. [2] performed 3-nucleotide saturation mutagenesis upstream of the -10 box and 4-nucleotide saturation mutagenesis within the -10 box to further expand the promoter library. | + | Li et al. [1] analyzed the proteomic characteristics of <i>Halomonas</i> TD01 using SDS-PAGE. They identified <B>a strongly expressed endogenous gene encoding a porin and preliminarily determined its promoter region</B>. Furthermore, they <b>identified the core region and constructed a constitutive promoter library by randomizing the sequences between the -35 and -10 regions</b>. Shen et al. [2] <b>performed 3-nucleotide saturation mutagenesis upstream of the -10 box and 4-nucleotide saturation mutagenesis within the -10 box to further expand the promoter library</b>. |
We have only included the porin constitutive promoters that are relevant to our team. | We have only included the porin constitutive promoters that are relevant to our team. | ||
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===References=== | ===References=== | ||
Latest revision as of 03:02, 14 September 2024
porin58
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
Li et al. [1] analyzed the proteomic characteristics of Halomonas TD01 using SDS-PAGE. They identified a strongly expressed endogenous gene encoding a porin and preliminarily determined its promoter region. Furthermore, they identified the core region and constructed a constitutive promoter library by randomizing the sequences between the -35 and -10 regions. Shen et al. [2] performed 3-nucleotide saturation mutagenesis upstream of the -10 box and 4-nucleotide saturation mutagenesis within the -10 box to further expand the promoter library. We have only included the porin constitutive promoters that are relevant to our team.
References
[1]Li T, Li T, Ji W, et al. Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp[J]. Biotechnology Journal, 2016, 11(2): 219-227.
[2]Shen R, Yin J, Ye JW, Xiang RJ, Ning ZY, Huang WZ, Chen GQ. Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis. ACS Synth Biol. 2018 Aug 17;7(8):1897-1906. doi: 10.1021/acssynbio.8b00102. Epub 2018 Jul 31. PMID: 30024739.