Difference between revisions of "Part:BBa K5226039"

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===Sequence and Features===
 
===Sequence and Features===
 
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===Introduction===
 
===Introduction===
 
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Revision as of 02:53, 14 September 2024


cysNC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 532
    Illegal SapI.rc site found at 600

Introduction


The existing methods for large-scale production of P34HB primarily rely on microbial fermentation. A key limiting factor in this process is the molar ratio of 4HB. Increasing the 4HB molar ratio can lead to a decrease in the melting temperature and apparent fusion heat of the copolymer, as well as an improvement in the polymer's deformation resistance. Therefore, enhancing the molar ratio of 4HB is crucial for the modification of P34HB.

Usage and Biology


cysNC is multifunctional fusion protein.The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the synthesis of activated sulfate and the synthesis of activated sulfate.In addition, it can forms the ATP sulfurylase (ATPS) with CysD to catalyze the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway.