Revision as of 13:46, 13 September 2024

4xMREd promoter

Usage and Biology

To achieve a cell-based heavy metal sensor, our group generated multiple synthetic MTF1 responsive promoter constructs and tested them in regard to their efficiency. Since Wang and colleagues (2004) suggested a high affinity of MTF-1 towards MREd we synthesized a promoter sequence containing four MREd elements at the positioning of the MREs of the MREwt promoter (K5317003) to exclude possible disruption of the MTF-1 and MRE interaction.

Cloning

Theoretical Part Design

The sequence of the MREd site was obtained from Seguin and colleagues (1987), as well as the positioning and spacer sequences (Searle et al. 1985). The construct was synthesized.

Sequence and Features

References