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Revision as of 13:43, 13 September 2024


MREdada promoter

Usage and Biology

The MRE-sites containing promoter enables the metal-dependent expression of a downstream positioned reporter gene via the metal ion-dependent transcription factor MTF-1 for cell-based metal detection.

To combine the observations made by Searle and colleagues and Wang and colleagues in 1985 and 2004, respectively, regarding the metal inducibility of a promoter with two MREa sites and a high affinity between MREd and MTF-1, we designed a synthetic promoter with two MREa and two MREd sites that alternate. The aim is possibly increasing the sensitivity and efficiency of the metal-dependent promoter.

Cloning

Theoretical Part Design

The MREdada promoter sequence was synthesized in order to insert it into the EGFP-C2 backbone (registry entry K3338020) for promoter efficiency testing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References