Difference between revisions of "Part:BBa K191003:Design"

 
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<partinfo>BBa_K191003 short</partinfo>
 
<partinfo>BBa_K191003 short</partinfo>
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===Source===
 
===Source===
  
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See subparts' references
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BBa_R0010, BBa_B0030, BBa_B0015.
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Plasmid containing LovTAP gene was kindly provided by the authors of : Light-activated DNA binding in a designed allosteric protein; Strickland et al., Proceedings of the National Academy of Sciences (2008) vol. 105 (31) pp. 10709
  
 
===References===
 
===References===

Revision as of 12:13, 21 October 2009

Promoter Lac I - RBS - LovTAP - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 764


Design Notes

2 restriction sites of Pst1 in LOVTAP


Source

BBa_R0010, BBa_B0030, BBa_B0015. Plasmid containing LovTAP gene was kindly provided by the authors of : Light-activated DNA binding in a designed allosteric protein; Strickland et al., Proceedings of the National Academy of Sciences (2008) vol. 105 (31) pp. 10709

References